Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification

Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification

Blog Article

Abstract In recent AC Drive Motor studies, electronegative multimodal chromatography with Eshmuno HCX was demonstrated to be a highly promising recovery step for direct immunoglobulin G (IgG) capture from undiluted cell culture fluid.In this study, the binding properties of HCX to IgG at different pH/salt combinations were systematically studied, and its purification performance was significantly enhanced by lowering the washing pH and conductivity after high capacity binding of IgG under its optimal conditions.A single polishing step gave an end-product with non-histone host cell protein (nh-HCP) below 1 ppm, DNA less than 1 curve-novelties ppb, which aggregates less than 0.5% and an overall IgG recovery of 86.2%.

The whole non-affinity chromatography based two-column-step process supports direct feed loading without buffer adjustment, thus extraordinarily boosting the overall productivity and cost-savings.